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MP102-LABINABAG: a prototype molecular diagnostic kit
MP102-LABINABAG: a prototype molecular diagnostic kit

MP102-LABINABAG: a prototype molecular diagnostic kit

The early diagnosis of plant pathogens from plant and soil samples needs a simple, rapid and cost-effective test, without the use of sophisticated and expensive equipment and reagents. In this study, the soil-borne pathogen Macrophomina phaseolina is used to demonstrate the potential for on-site molecular detection of the prototype diagnostic kit developed. This was achieved using a rapid and simple protocol comprising of a magnetic bead-based nucleic acid extraction, a portable PCR platform and a final NALFIA assay with a generic lateral flow device (LFD). The portable PCR platform powered by an external battery pack and all materials and reagents were included in a professional bag.


Italy


MP102-LABINABAG: a prototype molecular diagnostic kit

Prof. Claudio Pugliesi: Professore associato Genetica Agraria, Dr. Marco Fambrini: Tecnico di laboratorio Genetica Agraria, Sig.ra Grazia Puntoni: Tecnico di laboratorio Patologia Vegetale, Dott.ssa Alessia Vinci: Laurea magistrale in Biotecnologie Vegeta

Dr. Susanna Pecchia: Assistant Professor Plant Pathology.
Prof. Claudio Pugliesi: Associate Professor Plant Genetics and Genomics.
Dr. Marco Fambrini: Laboratory technician Plant Genetics and Genomics
Mrs. Grazia Puntoni: Laboratory technician Plant Pathology
Dr. Alessia Vinci: Master degree in Plant and Microbial Biotechnologies
Dr. Daniele Da Lio: Master degree in Plant and Microbial Biotechnologies


Stand A10 (pav. 7) - Università di Pisa


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Data updated on 2019-10-20 - 5.32.19 pm